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Knockdown of miR-299-5p inhibits the progression of hepatocellular carcinoma by targeting SIAH1 / Xinghua Jiang in Bulletin du cancer, vol. 105, 10 (Octobre 2018)
[article]
in Bulletin du cancer > vol. 105, 10 (Octobre 2018) . - p. 873-883
Titre : Knockdown of miR-299-5p inhibits the progression of hepatocellular carcinoma by targeting SIAH1 Type de document : article de périodique Auteurs : Xinghua Jiang ; Xiaoxu Shen Année de publication : 2018 Article en page(s) : p. 873-883 Note générale : Doi : 10.1016/j.bulcan.2018.07.013 Langues : Français (fre) Descripteurs (mots clés) : [Thésaurus Mesh]:C:Carcinome hépatocellulaire:Carcinome hépatocellulaire / génétique
[Thésaurus Mesh]Biologie cellulaire
[Thésaurus Mesh]Marqueurs biologiques
[Thésaurus HELB]:Paramédical:Micro-ARN MIRN299, humainMots-clés : Carcinome hépatocellulaire / génétique Biologie cellulaire Marqueurs biologiques Micro-ARN MIRN299, humain Seven in absentia proteins SIAH1 Résumé : Background
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. MiR-299-5p has been demonstrated to play important roles in multiple human cancers. Nevertheless, little is known about the detailed function and molecular mechanism of miR-299-5p on HCC progression.
Methods
Quantitative real-time PCR (qRT-PCR) assay was used to assess the expression patterns of miR-299-5p and siah E3 ubiquitin protein ligase 1 (SIAH1) in HCC tissues and cell lines. Loss-of-function experiments were performed to explore the effect of miR-299-5p on HCC progression in vitro and in vivo. Target predicted by software algorithms, the connection between miR-299-5p and SIAH1 was verified by dual-luciferase reporter assay, qRT-PCR and western blot analysis. Subsequently, anti-miR-299-5p and si-SIAH1 were cotransfected into LM9 and Huh-7 cells to further explore whether the regulatory effect of miR-299-5p on HCC was mediated by SIAH1.
Results
qRT-PCR assay revealed that miR-299-5p was upregulated and SIAH1 was downregulated in HCC tissues and cell lines. Moreover, miR-299-5p knockdown suppressed HCC progression in vitro and in vivo. In addition, anti-miR-299-5p-mediated regulatory effect on HCC cells was abated following the restoration of SIAH1 expression.
Conclusions
MiR-299-5p knockdown suppressed the progression of HCC by targeting SIAH1, highlighting its role as a potential biomarker and therapeutic target of HCC.Permalink : https://bibliotheque.helb-prigogine.be/opac_css/index.php?lvl=notice_display&id= [article]Exemplaires
Cote Support Localisation Section Disponibilité B Périodique Erasme - périodiques Périodiques Disponible A single nucleotide variant in microRNA-1269a promotes the occurrence and process of hepatocellular carcinoma by targeting to oncogenes SPATS2L and LRP6 / Pei Min in Bulletin du cancer, vol. 104, 4 (Avril 2017)
[article]
in Bulletin du cancer > vol. 104, 4 (Avril 2017) . - p. 311-320
Titre : A single nucleotide variant in microRNA-1269a promotes the occurrence and process of hepatocellular carcinoma by targeting to oncogenes SPATS2L and LRP6 Type de document : article de périodique Auteurs : Pei Min ; Wenshuai Li ; Fen Tang ; [et al.] Année de publication : 2017 Article en page(s) : p. 311-320 Langues : Anglais (eng) Descripteurs (mots clés) : [Thésaurus Mesh]:C:Carcinome hépatocellulaire:Carcinome hépatocellulaire / génétique Mots-clés : Carcinome hépatocellulaire / génétique miR-1269a LRP6 rs73239138 Résumé : Hepatocellular carcinoma (HCC) is one of the malignant and lethal cancers. Single nucleotide polymorphisms (SNPs) in microRNAs(miRNAs) can affect the expression and target identification of miRNAs and lead to the formation of malignant tumors. However, little is known about whether microRNA-1269a (miR-1269a) SNPs affect the susceptibility and progression of HCC or their specific mechanism. The association between microRNA-1269a rs73239138 and the susceptibility to HCC was verified by MassARRAY assay in a large case-control sample. The effect of miR-1269a and the variant on the proliferation and apoptosis of HCC cells was examined by flow cytometry (FCM), CCK8 assay and Western blot. The target of miR-1269a was identified by bioinformatics analysis and qRT-PCR and its role on cell proliferative capacity was examined by CCK8 assay. The expression level of miR-1269a was analyzed by qRT-PCR in HCC cells transfected with wild or variant type pre-miR-1269a plasmid.MiR-1269a produced a tumor suppressor effect by inhibiting cell proliferation and inducing apoptosis of human HCC cells, possibly via inhibiting the expression of its target genes SPATS2L and LRP6, which were tumor promoters. While, rs73239138 (G>A) in miR-1269a reduced the anticancer effect of miR-1269a possibly by attenuating its total amount in HCC cells or its target recognition, reduce its inhibition on target genes and promoted the susceptibility to HCC. Our findings for the first time proved that miR-1269a SNP plays a role in the occurrence and process of HCC and the relevant mechanism, in accompany with the discovery of the novel target genes of miR-1269a. Permalink : https://bibliotheque.helb-prigogine.be/opac_css/index.php?lvl=notice_display&id= [article]Exemplaires
Cote Support Localisation Section Disponibilité B Périodique Erasme - périodiques Périodiques Disponible