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Exosomes
Vesicles secreted from MULTIVESICULAR BODIES into the extracellular environment when the multivesicular bodies fuse with the PLASMA MEMBRANE. Multivesicular bodies are formed from ENDOSOMES when they accumulate vesicles (sometimes referred to as "intraluminal vesicles") from inward budding of the endosome membrane.
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Colorectal cancer cell–derived exosomes containing miR-10b regulate fibroblast cells via the PI3K/Akt pathway / Guangyao Dai in Bulletin du cancer, vol. 105, 4 (Avril-juin 2018)
[article]
in Bulletin du cancer > vol. 105, 4 (Avril-juin 2018) . - p. 336-349
Titre : Colorectal cancer cell–derived exosomes containing miR-10b regulate fibroblast cells via the PI3K/Akt pathway Type de document : article de périodique Auteurs : Guangyao Dai ; Xiaoguang Yao ; Yubin Zhang Année de publication : 2018 Article en page(s) : p. 336-349 Note générale : https://doi.org/10.1016/j.bulcan.2017.12.009 Langues : Français (fre) Descripteurs (mots clés) : [Thésaurus Mesh]:T:Tumeurs colorectales:Tumeurs colorectales / physiopathologie
[Thésaurus Mesh]Carcinogenèse
[Thésaurus Mesh]Exosomes
[Thésaurus Mesh]Fibroblastes associés au cancer
[Thésaurus Mesh]MicroARNMots-clés : Fibroblastes associés au cancer Tumeurs colorectales / physiopathologie Carcinogenèse Exosomes MicroARN MiR-10b PIK3CA PI3K/Akt/mTOR pathway Résumé : Background
Cancer-associated fibroblasts (CAFs) contribute to the proliferation of colorectal cancer(CRC) cells. However, the mechanism by which CAFs develop in the tumor microenvironment remains unknown. Exosomes may be involved in activating CAFs.
Methods
Using a miRNA expression profiling array, we determined the miRNA expression profile of secretory exosomes in CRC cells and then identified potential miRNAs with significant differential expression compared to normal cells via enrichment analysis. Predicted targets of candidate miRNAs were then assessed via bioinformatics analysis. Realtime qPCR, western blot, and cell cycle analyses were performed to evaluate the role of candidate exosomal miRNAs. Luciferase reporter assays were applied to confirm whether candidate exosomal miRNAs control target pathway expression. A CRC xenograft mouse model was constructed to evaluate tumor growth in vivo.
Results
Exosomes from CRC cells contained significantly higher levels of miR-10b than did exosomes from normal colorectal epithelial cells. Moreover, exosomes containing miR-10b were transferred to fibroblasts. Bioinformatics analysis identified PIK3CA, as a potential target of miR-10b. Luciferase reporter assays confirmed that miR-10b directly inhibited PIK3CA expression. Co-culturing fibroblasts with exosomes containing miR-10b significantly suppressed PIK3CA expression and decreased PI3K/Akt/mTOR pathway activity. Finally, exosomes containing miR-10b reduced fibroblast proliferation but promoted expression of TGF-? and SM ?-actin, suggesting that exosomal miR-10b may activate fibroblasts to become CAFs that express myofibroblast markers. These activated fibroblasts were able to promote CRC growth in vitro and in vivo.
Conclusion
CRC-derived exosomes actively promote disease progression by modulating surrounding stromal cells, which subsequently acquire features of CAFs.Permalink : https://bibliotheque.helb-prigogine.be/opac_css/index.php?lvl=notice_display&id= [article]Exemplaires
Cote Support Localisation Section Disponibilité BUL Périodique Erasme - périodiques Périodiques Disponible Exosomes of glioma cells deliver miR-148a to promote proliferation and metastasis of glioblastoma via targeting CADM1 / Li Gong in Bulletin du cancer, vol. 105, 7-8 (Juillet-août 2018)
[article]
in Bulletin du cancer > vol. 105, 7-8 (Juillet-août 2018) . - p. 643-651
Titre : Exosomes of glioma cells deliver miR-148a to promote proliferation and metastasis of glioblastoma via targeting CADM1 Type de document : article de périodique Auteurs : Li Gong ; Anding Zhu ; Qian Cai, Auteur Année de publication : 2018 Article en page(s) : p. 643-651 Note générale : https://doi.org/10.1016/j.bulcan.2018.05.003 Langues : Français (fre) Descripteurs (mots clés) : [Thésaurus Mesh]Biologie moléculaire
[Thésaurus Mesh]Exosomes
[Thésaurus Mesh]Glioblastome
[Thésaurus Mesh]Métastase tumoraleMots-clés : Glioblastome Métastase tumorale Biologie moléculaire Exosomes cellules de gliome miR-148a Prolifération CADM1 Résumé : Exosomes are now considered to be involved in mediating cell-to-cell communication to promote or inhibit tumor progression. However, the role and molecular mechanism of exosomes in promoting glioblastoma (GBM) metastasis remains elusive. Here, we found that circulating exosomal miR-148a levels were significantly higher in serum from GBM patients compared with serum from healthy volunteers. In T98G cells, inhibition of miR-148a suppressed cell proliferation and metastasis. In addition, we identified Cell adhesion molecule 1 (CADM1) as a target gene of miR-148a using luciferase reporter assay. Both protein and mRNA levels of CADM1 were decreased in tissues from GBM patients. There was a strong negative correlation between exosomal miR-148a and CADM1 mRNA levels in samples of patients. Moreover, miR-148a antagonist increased p-STAT3 protein level to activate STAT3 pathway. In conclusion, our findings indicated that miR-148a delivered by exosomes may promote cancer cell proliferation and metastasis via targeting CADM1 to activate STAT3 pathway, suggesting a predictor and therapeutic target role of exosomal miR-148a in GBM patients. Permalink : https://bibliotheque.helb-prigogine.be/opac_css/index.php?lvl=notice_display&id= [article]Exemplaires
Cote Support Localisation Section Disponibilité B Périodique Erasme - périodiques Périodiques Disponible