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Evaluation of Polymerase Chain Reaction over Routine Microbial Diagnosis for the Diagnosis of Fungal Keratitis / Himanshu Sekhara Behera in OVS : Optometry & Vision Science, vol. 98, 03 (Mars 2021)
[article]
in OVS : Optometry & Vision Science > vol. 98, 03 (Mars 2021)
Titre : Evaluation of Polymerase Chain Reaction over Routine Microbial Diagnosis for the Diagnosis of Fungal Keratitis Type de document : article de périodique Auteurs : Himanshu Sekhara Behera ; Deepankar Srigyan Année de publication : 2021 Langues : Français (fre) Descripteurs (mots clés) : [Thésaurus Mesh]Diagnostic
[Thésaurus Mesh]Kératite
[Thésaurus HELB]:Paramédical:Réservoirs de maladiesMots-clés : kératite fongique kératite mycosique Résumé : SIGNIFICANCE: The significance of the study is that, although conventional culture remains the criterion standard
for identifying the causative fungal pathogens, polymerase chain reaction (PCR) may serve as a powerful and
high-throughput tool for the early and definitive diagnosis of high-risk patients with mycotic keratitis owing to high
sensitivity and specificity.
PURPOSE: This study was focused on comparing the results of PCR with traditional microbial studies for the detection and identification of fungal pathogens in patients with clinically suspected fungal keratitis.
METHODS: Corneal scrapings were collected from 59 patients with clinically suspected fungal keratitis for routine
culture, staining, and seminested PCR assay for fungal pathogen identification. The results of PCR were compared
with a conventional microbial workup (smear and culture). The samples that were unidentified by culture but were
amplified by PCR were further identified by nucleotide sequencing.
RESULTS: Of the 59 patients with suspected fungal keratitis, 38 (64.40%) were found to be positive by PCR assay, 24 (40.67%) by culture, 18 (20.3%) by potassium hydroxide wet mount, and 8 (13.5%) by Gram stains for
fungal pathogens. All the 24 isolates found positive with culture were also positive with PCR, so they had not been
sequenced for molecular identification. The remaining 14 isolates that were positive with PCR but negative with
culture were further identified as Cladosporium cladosporioides, Simplicillium species, Fusarium solani, Alternaria
tenuissima, Chaetomium globosum, Penicillium citrinum, and Rhizopus microsporus by sequencing up to the species level.
CONCLUSIONS: The PCR was able to detect the presence of fungal pathogens in a high proportion of culture-negative
cases. This study suggests that PCR may serve as a rapid, important complement to traditional culture with
high-throughput means of fungal pathogen identification in patients with clinically suspected fungal keratitis.Permalink : https://bibliotheque.helb-prigogine.be/opac_css/index.php?lvl=notice_display&id= [article]Exemplaires
Cote Support Localisation Section Disponibilité aucun exemplaire Hurricane Harvey Aftermath: An Interdisciplinary Case Report on the Management of an Open Bimalleolar Fracture / Rosemary Buckle in Journal of the American Podiatric Medical Association, vol. 110, 02 (Mars-avril 2020)
[article]
in Journal of the American Podiatric Medical Association > vol. 110, 02 (Mars-avril 2020)
Titre : Hurricane Harvey Aftermath: An Interdisciplinary Case Report on the Management of an Open Bimalleolar Fracture Type de document : article de périodique Auteurs : Rosemary Buckle ; Anum Dhukani ; Rayven L. Bridges ; Barton C. Garcia Année de publication : 2020 Langues : Anglais (eng) Descripteurs (mots clés) : [Thésaurus HELB]:Paramédical:prise en charge
[Thésaurus HELB]:Paramédical:Réservoirs de maladies
[Thésaurus Mesh]Fractures de la cheville
[Thésaurus Mesh]Fractures ouvertes
[Thésaurus Mesh]Infections de la peau
[Thésaurus Mesh]Présentations de cas
[Thésaurus Mesh]Relations interprofessionnelles
[Thésaurus Mesh]Tempêtes cycloniquesRésumé : Natural disasters, such as hurricanes and severe flooding, pose a threat of increased skin and soft-tissue infections, especially in the event of open fractures and wading through the waters. The purpose of this case study is to present a complex patient sustaining trauma resulting in an open bimalleolar fracture, multiple wounds, and exposure to a variety of water-borne pathogens during Hurricane Harvey in Houston, Texas, in 2017. He underwent multiple incision and drainage procedures, tissue cultures, and placement of antibiotic beads, with an application of external fixation to the left ankle. Several unique multidrug-resistant water-borne pathogens were identified, including Aeromonas hydrophila, Pseudomonas fluorescens/putida, and Serratia marcescens. Once the soft-tissue envelope was restored and infection cleared, a full-thickness rotational flap with tissue expansion was performed. Ultimate reconstruction was delayed several weeks and final left ankle open reduction and internal fixation was performed following antimicrobial treatment with split-thickness skin autograft and wound vacuum-assisted closure application. The patient was discharged after 28 days with no further complications. In instances such as these, all caretakers coming into contact with the patient should be aware of the potential risks of the possible infectious diseases and management to optimize the recovery following hydrologic disasters. Note de contenu : Rosemary Buckle, MD; Anum Dhukani, DPM; Rayven L. Bridges, DPM; Barton C. Garcia, DPM Permalink : https://bibliotheque.helb-prigogine.be/opac_css/index.php?lvl=notice_display&id= [article]Exemplaires
Cote Support Localisation Section Disponibilité aucun exemplaire