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Auteur Rachel L. Redfern
Documents disponibles écrits par cet auteur
Ajouter le résultat dans votre panier Affiner la rechercheImpact of Low Humidity on Damage-associated Molecular Patterns at the Ocular Surface during Dry Eye Disease / Alyce Alven in OVS : Optometry & Vision Science, vol. 98, 11 (Novembre 2021)
[article]
in OVS : Optometry & Vision Science > vol. 98, 11 (Novembre 2021)
Titre : Impact of Low Humidity on Damage-associated Molecular Patterns at the Ocular Surface during Dry Eye Disease Type de document : article de périodique Auteurs : Alyce Alven ; Carolina Lema ; Rachel L. Redfern Année de publication : 2021 Langues : Français (fre) Descripteurs (mots clés) : [Thésaurus Mesh]:S:Syndromes de l'oeil sec:Syndromes de l'oeil sec / complications
[Thésaurus Mesh]Cytokines
[Thésaurus Mesh]Inflammation
[Thésaurus Mesh]Syndromes de l'oeil secRésumé : SIGNIFICANCE
Dry eye is one of the leading causes for individuals to seek eye care, whereas the pathogenesis is poorly understood. One mechanism in which dry eye inflammation may ensue is by the release of damage-associated molecular patterns (DAMPs) by damaged cells to stimulate the production of cytokines and matrix metalloproteinases. Examining DAMP levels on the ocular surface during dry eye disease (DED) will increase our understanding of their potential involvement in the pathogenesis of DED.
PURPOSE
This study aimed to quantitate DAMPs, high-mobility group box 1 (HMGB1), and heat shock proteins on the ocular surface of normal and dry eye subjects and to examine the impact of low-humidity environment (LHE) on DAMPs and inflammation in dry eye subjects.
METHODS
Basal tears (10 to 20 μL) and conjunctival impression cytology samples were analyzed for HMGB1, HSP-27, HSP-60, HSP-70, and HSP-90α by ELISA or Luminex assays in normal (n = 15) and DED (n = 15) subjects. In addition, a subset of DED subjects were exposed to LHE for 2 hours. The level of DAMPs in the tear film was evaluated by ELISA or Luminex assay. Interleukin 6, interleukin 8, or metalloproteinase (MMP) 9 mRNA were quantitated by real-time polymerase chain reaction from conjunctival impression cytology samples.
RESULTS
Compared with age-matched normal subjects, HMGB1 was significantly elevated in the tear film of DED subjects (P = .03), whereas there was no significant difference in heat shock proteins. Conjunctival impression cytology samples revealed no significant difference in intracellular DAMP levels between both groups. After exposure to an LHE, there was an increase in corneal staining (P = .005), HSP-60 levels in the tear film (P = .01), and MMP-9 mRNA in the conjunctiva (P = .001).
CONCLUSIONS
Dry eye subjects had higher levels of HMGB1 in their tear film. Exposure to an LHE worsened corneal staining, increased conjunctival MMP-9 mRNA expression, and increased tear film HSP-60 levels. Larger studies are needed to understand the involvement of DAMPs in stimulating dry eye inflammation.Permalink : https://bibliotheque.helb-prigogine.be/opac_css/index.php?lvl=notice_display&id= [article]Exemplaires
Cote Support Localisation Section Disponibilité aucun exemplaire IOP and Optic Nerve Head Morphology during Scleral Lens Wear / Maria K. Walker in OVS : Optometry & Vision Science, vol. 97, 09 (Septembre 2020)
[article]
in OVS : Optometry & Vision Science > vol. 97, 09 (Septembre 2020)
Titre : IOP and Optic Nerve Head Morphology during Scleral Lens Wear Type de document : article de périodique Auteurs : Maria K. Walker ; Laura Pardon ; Rachel L. Redfern ; Nimesh Patel Année de publication : 2020 Langues : Français (fre) Descripteurs (mots clés) : [Thésaurus HELB]:Optique:port de lentilles de contact
[Thésaurus HELB]:Optique:pression intraoculaire
[Thésaurus HELB]:Paramédical:Compression
[Thésaurus HELB]:Paramédical:Lentille sclérale
[Thésaurus Mesh]Nerf optique
[Thésaurus Mesh]Tissu conjonctifRésumé : SIGNIFICANCE: Scleral lenses (SLs) are increasing in scope, and understanding their ocular health impact is imperative. The unique fit of an SL raises concern that the landing zone causes compression of conjunctival tissue
that can lead to resistance of aqueous humor outflow and increased intraocular pressure (IOP).
PURPOSE: This study aimed to assess changes in optic nerve head morphology as an indirect assessment of IOP
and evaluate other IOP assessment methods during SL wear.
METHODS: Twenty-six healthy adults wore SL on one randomly selected eye for 6 hours, whereas the fellow eye
served as a control. Global minimum rim width (optical coherence tomography) and IOP (Icare, Diaton) were measured at baseline, 2 and 6 hours after SL application, and again after SL removal. Central corneal thickness, anterior chamber depth, and fluid reservoir depth were monitored.
RESULTS: Minimum rim width thinning was observed in the test (−8 μm; 95% confidence interval [CI], −11 to
−6 μm) and control (−6 μm; 95% CI, −9 to −3 μm) eyes after 6 hours of SL wear (P < .01), although the magnitude
of thinning was not significantly greater in the lens-wearing eyes (P = .09). Mean IOP (Icare) significantly increased
+2 mmHg (95% CI, +1 to +3 mmHg) in the test eyes (P = .002), with no change in the control eyes. Mean IOP
changes with Diaton were +0.3 mmHg (95% CI, −0.9 to +3.2 mmHg) in the test eyes and +0.4 mmHg (95%
CI, −0.8 to +1.7 mmHg) in the control eyes. However, Diaton tonometry showed poor within-subject variation
and poor correlation with Icare. No clinically significant changes were observed in central corneal thickness or anterior chamber depth.
CONCLUSIONS: This study suggests that SLs have a minimal effect on IOP homeostasis in the normal eye during
SL wear and an insignificant impact on the optic nerve head morphology in healthy adult eyes.Permalink : https://bibliotheque.helb-prigogine.be/opac_css/index.php?lvl=notice_display&id= [article]Exemplaires
Cote Support Localisation Section Disponibilité aucun exemplaire